A High-Throughput Strategy for Glycine Oxidase Biosensor Development Reveals Glycine Release from Cultured Cells

Glycine is an important biomarker in clinical analysis due to its involvement multiple physiological processes. As such, the need for low-cost analytical tools glycine detection growing. a neurotransmitter, involved inhibitory and excitatory neurochemical transmission central nervous system. In this work, we present 10 μM Pt-based electrochemical enzymatic biosensor localized real time measurements of glycine. This relies on amperometric readout does not require additional redox mediators. The was characterized applied real-time from cells, mainly HEK 293 cells primary rat astrocytes. Taken together, study demonstrates oxidase based useful extracting pertinent information about levels wide range biological contexts.